Intro:
This lab involves us to perform "genetic tranformation" which invloves a pice of DNA which provides the instructions and it really means for us to change the genetic information. This entales us to take a gene and insert it into another organism inorder to change the organism's traits. The use of genetic transformation is is used in biomediation which is consisting of oilspill soaking bacteria and also it's used in agriculture. The use in agriculture is abundant and is used to prevent disease killing and to keep certain traits in plants to make a perfect crop. Its is in medicine as well, the diseases caused by defective genes are beginning to be treated by gene therapy by transformaing the sick persons cells with healthy coppies of the defactive gene to help with the disease.
The experiment we will be conducting involves us to transform bacteria with the gene with (GFP) or Green Fluorescent Protien which is in the sea jelly Aequorea Victoria. The bacteria will have this gene and express it under ultraviolet light and cause them to glow a brilliant green.
Using the plasmid we will learn how to be able to tranfer thes genes from one organism to the next and have a funn time doing it. The bacteria contain circular pieces of DNA called plasmids these contain genes for one or more of the organisms traits that could be important to the bacteria's survival.
A - you need to discss controls, variables, and procedure. Otherwise, very good!
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