The micro array chips is the most powerful new research tool that makes the viewing of thousands of genes expressed in cells. It is bess used for looking for patterns or changes in transcription in cells which makes the study of normal and abnormal of the cells function easier.
A singel micro array can contain more than 30,000 spots of DNa, each is representing a different genes in the organism. This chip is used to study the expression of six different genes in normal lung cells and lung cancer cells:
Gene 1
The protien that codes for this gene helps start our immune system to kill the pathogens
Gene 2
The gene helps code for an enzyme catalyzes the conversion of omithine to putrescine
Gene 3
Codes for a part of fibrinogen, a protien found in blood that helps in the forming of blood clots
Gene 4
One of the two y-globulin genes
Gene 5
Plays the role in inducing cell differentation, controlling cell cell growth
Gene 6
catalyze many of the reactions involved in drug metabolism and maing cholesterol, steroids, ande other lipids.
Procedure:
Take the micro array slide and useing a pippet spot on the 6 different gene sequences onto the glass slide
carefully add the 20mL of hybridization solution to each spot (Dont allow the dropper to touch the spot)
Record the results by weiting a discription of the color of each spot in your results.
CLEAN UP
Results/Observations:
#1 and #5 pink
transcribed from the DNA to mRNA-> cDNA (colored)
#3,#4,#6 no pink dye showed up
#4 a non protien coating gene (could be found prodice hemoglobin)
#2 is expressed in both which means no real change from normal to cancerous cells
#1 and #5 only expressed in cancerous cells (#1,#3#5.#6 all in cancer)
Discussion:
a) even though there wasnt a real way to hypothesize the out come the results were not as what we would have thought because we havent ever done DNA mirco arrays before.
b) When we did the color of one of the spots we didnt realize that it changed purple at the beginning so over time it turned blue.
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