The Diseased at Acalanes

For this lab we will be using the technique of PCR to extract the DNA to make it able to get he certain parts of the DNA we are searching for. In 1983, Karry Mullis at Cetus Corporation developed the molecular biology technique for this type of research which is called PCR. The reason we use PCR is that it produces a abundance of DNA in a test tube starting from a little bit of it. So basically it is a way of amplifying the DNA sequence, gene of interest. With this we can use the genomic DNA and just from a DNA sample no matter how small it can be made bigger amount so it has enough DNA to study with.

PCR is the best for genetic research, gene mapping, cloning, DNA sequencing, and gene detection and will be used in medical diagnostic. It will be a tool to detect the mutations that cause the genetic disease, also in criminal investigations and court of law to help identify the suspects, sequencing the human genome.



Procedure:

Day 1
Extract the cheek cells by chewing the in inside of your cheeks for 30 seconds
get the DNA and put the InstaGene Matrix inside of the small tube
put in the heat bath for 10 mins
heat then in the 94 degrees Celsius water bath
vortex the tube for 1 min
extract the water on the top of the tube and leave the clump on the bottom

Day 2
Add the loading dye to the DNA sample
and also add the controls of +/+ -/- +/- and marker to the gel in the last lane
add the DNA samples from the students samples
Run the gel *running to red for 15 mins
transfer gel when done to a dye stain tray
go to the back and add the blue stain to stain the DNA in the gel
put on rocker to marinade the gel over the weekend

Day 3 


Results:


i figured out what we were looking for was the having the so called "disease" and i ended up having the disease gene and only one person in our group was able to not have that gene.


Discussion:
a) i had no idea of what to hypothesis but we tested for the presence of an enzyme and the odds were high to have it show up as positive so i guessed i would be so called "diseased"

b) DONT chew too hard or else you will start to bleed from your cheeks and also make sure you do it long enough so you have enough cells.