Intro:
a) DNA is in all living things including bacteria. plants, animals and most cell types. The carrier of the genetic information consisting of hair, skin, and eye color, facial features and everything that hs to do with the appearance of people. It also carries info for the cells to do their normal function and are known as the "blueprints". This consists of your father's and your mother's DNA.
DNA looks the same in everything but seeing your DNA is cool, knowing that it is the info that constructs you. The extraction is simple and is done by scientists all the time from different organisms.
b) Everyday scientists use the DNA to make new discoveries through the code embedded in the strands. These new discoveries include cures for diseases tat could be the answer to the people suffering.
c) 15ml tube, saline solution, plastic cup, plastic pipette, cold alcohol.
d) The DNA when extracting will appear in a cloudy bunch on the top of the alcohol.
Procedure:
Get a 15 ml tube and put 3ml of saline solution in the tube.
gently chew the inside of the4 cheeks
take the solution and swish it in the mouth for 30 sec
spit the solution back into the cup and then put back into tube
get the tube and put 2ml of lysis buffer in it
put the cap on the tube and invert the tube 5 times
add 100 ml of protease
put cap back on and invert
place the tube in the 50 degree celsius bath for 10 mins
add the 10 ml of cold alcohol
let sit for 5 mins
after invert the tube to help the DNA gather
get the plastic pipette and extract the DNA
Results/Observations:
After adding the lysis buffer the liquid seemed to thicken and when we were done the DNA clouded up and gathered up into a ball.
Disussion:
a) The hypothesis we all got was as we thought would happen, we thought the DNA would form when the alcohol was put in the tube.
b) When we got the DNA there seemed to not have as much DNA as i thought there was going to be. I may have needed to chew on my cheeks a little more or better to have more cheek cells.
Excellent - A+
ReplyDelete